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Fig. 8. Interactions between duet and dyad during male meiosis
and pollen development. Optical sections of cleared anthers viewed under DIC
optics. (A-D) duet dyad double mutant; (E-L) duet/+
dyad/dyad. (A,E) Normal-looking meiocytes (arrowheads). (B) Defective
microspores lacking exine (arrowhead). (C) Binucleate spores. (D) Late stage
defective binucleate and multinucleate spores (arrow and arrowhead,
respectively) surrounded by an uneven wall resembling the exine; not observed
in the duet single mutant. (F) Dyad (arrowhead). (G) Undivided triads
(arrowhead). (H) Round spores containing 1-3 nuclei. (I) Normal-looking
tetrads (arrowhead). (J) Normal-looking developing pollen. (K) Abnormal
multinucleate structures undergoing shrinkage (arrowhead). (L) Shrunken
pollen. Scale bar: 12.5 µm.