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Fig. 2. Delayed and reduced production of Pdgfra + (A-H) and NG2+ (I-L)
OPCs in Nkx6.1 mutant spinal cords. Spinal cord sections from E13.5
(A,B), E15.5 (C,D,I,J), E17.5 (E,F) and P0 (G,H,K,L) wild-type (A,C,E,G,I,K)
and mutant (B,D,F,H,J,L) embryos were subjected to in situ hybridization with
Pdgfra riboprobe or double immunofluorescence with anti-NG2 (red) and
anti-PECAM1 (green) antibodies. During embryogenesis, NG2 labels both OPC
cells and PECAM1+ immature blood vessels
(Ozerdem et al., 2001).