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Fig. 8. Multiple sites of origin of Olig1 and Olig2 expression in the rostral hindbrain during early stages of oligodendrogenesis. (A-H) Adjacent slide sections from E13.5 wild-type (A,C,E,G) and mutant (B,D,F,H) hindbrain at the pontine area were hybridized with Olig1 (A,B), Olig2 (C,D), Pdgfra (E,F) and Nkx2.2 (G,H) probes. Only the tissue staining surrounding the fourth ventricle is shown. In A-D, arrows indicate Olig1 and Olig2 expression in the Nkx6.1/Nkx2.2 domain of the ventral ventricular zone; arrowheads indicate Olig1/2 expression outside the Nkx6.1 domain. (I,J) Slide sections from E13.5 wild-type hindbrain at the same level were double-labeled with Nkx6.1 (blue) and Olig1 (orange), or Nkx2.2 (blue) and Olig1 (orange). In I, the blue arrow indicates the dorsal boundary of Nkx6.1 expression.





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