Fig. 2. Asymmetric expression of leftover in the habenular nuclei. (A) The left habenula (white arrowhead) expresses lov (blue) at higher levels and in more cells than the right. (B) Asymmetric lov expression persists in the adult zebrafish brain. (C-E) lov expression in the left habenula coincides with a region of dense neuropil (black arrowhead on left side in D, compare with right side) labeled with anti-acetylated tubulin antibody. (F) The lov gene encodes a 288 amino acid protein that contains a conserved oligomerization domain (T1-like domain) at the N-terminus. (G) The Leftover oligomerization domain is 47% similar to the T1 tetramerization domain of the Drosophila Shaker K⁺ channel (Papazian, 1999) and 41% similar (not shown) to the POZ/BTB domain of human promyelocytic leukemia zinc finger protein (PLZF) (Collins et al., 2001). Scale bars: 50 µm for A,C-E; 150 µm for B.

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