Fig. 6. Phox2b-expressing progenitors of visceral and branchial motoneurons in hindbrain of E10.5 wild-type and Nkx2.9 mutant mouse embryos. Transverse sections through hindbrain at the level of rhombomere 4 (A,B,E,F) and rhombomere 7 (C,D,G,H) were immunostained with Phox2b-(A,E,C,G) and Isl1-specific antibodies (B,F,D,H). Sections of wild-type (A-D) and mutant embryos (E-H) reveal similar patterns of Phox2b expression in mitotic and postmitotic cells of the ventral and lateral domain, and in the mantel layer of the dorsal domain at r4 level. At r7 only postmitotic cells of the ventral domain and a dorsoventral stripe of presumably migratory cells express Phox2b. Isl1-positive cells are found only in postmitotic cells of the ventral domain both at r4 and r7 levels. Note that the expression of both markers appears essentially unchanged in wild-type and mutant embryos.

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