Fig. 2. dri is expressed in longitudinal glial cells of the Drosophila embryonic CNS. Confocal micrographs of stage 15 whole-mount embryos. Embryos are immunochemically stained with polyclonal rabbit anti-ß-galactosidase (Alexa488-conjugated secondary antibody, green) and rat anti-DRI (Lissamine-Rhodamine-conjugated secondary antibody, red). Anterior is towards the left. (A,B) Focal planes showing that dri is expressed in the medialmost cell body glia (MM CBG, arrow in A), the subperineural glia (SPG, double arrowheads in B) and longitudinal glial (LG, short arrow in B) that express ß-galactosidase in the rA87 enhancer trap line. (C) dri is expressed in the A and B SPG (double arrowhead) and LG (short arrow) that express ß-galactosidase in the pnt^rm254 enhancer trap line. (D) dri is expressed in the A and B SPG (double arrowheads) and LG (short arrow) that express ß-galactosidase in the rC56 enhancer trap line. (E) dri is expressed in the A and B SPG (double arrowhead) and LG (short arrow) that express repo. (F) dri is expressed in all six prospero-expressing LG (short arrows, only five of these cells are in focus). (G) ct and dri are co-expressed in the LG (short arrow). (H) CNS stained for DRI alone showing the assignments of cells derived from the data shown in (A-F): LG (short arrow), A and B SPG (double arrowheads), MM CBG (long arrow) and N (neurone, notched arrowhead).

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