Fig. 8. The number of cells expressing loco and pros is reduced in dri mutants. Dorsal view of dissected ventral nerve cord preparations of dri/CyO wgß gal (A,C,E) and dri¹/dri² (B,D,F) stage 16 embryos, immunochemically stained with anti-PROS and anti-ß-galactosidase (A-D), anti-ß-galactosidase and anti-DRI antibodies (E,F) and detected with AP-conjugated (for PROS and rC56 ß-gal; A-F) and, in some cases, HRP-conjugated (for ß-gal: A,C; and DRI in E) secondary antibody. Anterior is towards the left. (A) In dri/CyO embryos there are five pros-positive cells in the focal plane shown here. (B) In dri mutant embryos, pros expression is absent in one cell per hemineuromere, depicted by an arrow. (C,D) Higher magnifications of A,B, respectively, showing that the four remaining pros-positive cells are not positioned in a flat sheet as they are in wild type. (E,F) rC56-directed ß-galactosidase expression is reduced or absent in the longitudinal glia of dri mutant embryos (arrows in F).

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