Fig. 4. Summary of data from the growth cone turning assay using ephrin-A5-coated beads and laser tweezers. Ephrin-A5-coated or control beads were placed (using laser tweezers) in front of the retinal growth cones. After contact with ephrin-A5-coated beads, roughly half of the observed growth cones showed growth cone collapse (7 of 15; 47%), while the other half reacted with repulsion (7 of 15; 47%). One growth cone did not show any reaction. Control beads (uncoated or coated with protein A) did not elicit any change in growth cone behaviour in most cases (10 of 12; 83%). However, two growth cones showed a growth cone collapse. In all experiments laminin was used as an outgrowth promoting substratum.

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