Fig. 2. At E14.5, abnormal ß1D1D ki/ki embryos showed defective vascularisation and increased apoptosis of endothelial cells in the placental labyrinth. (A,D,G) Heterozygous, (J) wild-type (B,E,H,K) normal ß1D1D ki/ki and (C,F,I,L) abnormal ß1D1D ki/ki embryos. (A-C) Laminin immunostaining, in transverse sections of E14.5 placentae, showing the basal lamina of foetal blood vessels. (D-F) Higher magnification of the labyrinth in A-C showing reduced branching and obstructed blood vessels in abnormal ß1D1D ki/ki embryos (arrow). (G-I) Haematoxylin and Eosin staining. (J-L) Transverse sections showing MECA32- (red) and TUNEL- (green) positive cells (arrows in L). cp, chorionic plate; lb, labyrinth. Scale bar: 200 µm.

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