Fig. 5. Gene expression in ES cells and in individual cloned embryos from ES cell nuclei. Oct4 and Oct4-related genes are abundantly expressed in the donor ES cells and in ES cell nuclei-derived clones. Note that although Dppa1 gene is strongly expressed in ES cells, it is initially downregulated in ES cell cloned morulae before re-activation in the blastocyst stage ES clones. This pattern of Dppa1 expression is reminiscent of its expression during normal embryogenesis (see Fig. 2). A full summary of gene expression is shown in Fig. 4.

Return to article