Fig. 6. Monitoring of Nodal activity in mouse embryos with a Nodal-responsive transgene. Expression of the Nodal-responsive transgene (n2)7-lacZ was examined in wild-type (WT) (A,B) and Foxh1^c/- (C,D) embryos. In the Foxh1^c/- embryo, X-gal staining in left LPM and PFP is lost whereas that in the allantois remains (C,D). Expression vectors for Nodal, EGFP and lacZ were also introduced into the left LPM of a Foxh1^c/- embryo harboring the (n2)7-lacZ transgene and, 6 hours later, the embryo was stained with X-gal (E,F). The region that received the expression vectors is apparent from the EGFP fluorescence and X-gal staining in left LPM (indicated by the square brackets). X-gal-positive region in left LPM failed to expand due to the absence of Foxh1 in LPM. A magnified view of the boxed region indicated in E is shown in F. The arrowhead in F indicates X-gal staining in the PFP that was induced by Nodal. Anterior views are shown in A,C,E, whereas left lateral views are shown in B,D.

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