Fig. 8. Sensory and motor axon pathfinding is relatively normal in Rac1V12 embryos. repo:GAL4 embryos carrying the UAS-actin-GFP marker are the wild type (A,B). repo::actin-GFP flies were crossed to a UAS-Rac1V12 transgenic construct line (C,D). Embryonic progeny were double labeled for glial Actin-GFP (green), sensory neurons (A,C; red, mAb 22C10) and motoneurons (B,D; red, mAb 1D4). Anterior is towards the top, CNS is towards the left. Stage 16 embryos are shown. (A) In the wild type, sensory axons (red) project into the CNS along two main fascicles (arrows). (C) In Rac1V12 embryos, the glial processes are collapsed. Sensory axons still pathfind such that they form two main fascicles as in the wild type (arrows); however, they are defasciculated (right arrow). (B) The SNa motor axon branch of a wild-type embryo is indicated (arrow). (D) In Rac1V12 embryos, pathfinding of the SNa motoneuron is essentially normal (arrow), as are other motor branches (not indicated).

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