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Fig. 2. The anchor cell (AC)-specific enhancer element of lin-3. (A) The
wild-type, but not`G to A', form of ACEL can activate
pes-10::gfp transcription in the AC. A base change from
guanine (G) to adenine (A) was identified in the lin-3(e1417) allele
(`G to A'). The wild-type and`G to A' forms of the putative
ACEL elements (59 bp) were cloned into a pes-10::gfp enhancer
assay vector. The number in parenthesis represents the number of animals in
which gfp expression in the AC was examined. (B) Conservation of the
ACEL sequence between C. briggsae (CB) and C. elegans (CE).
The intron sequence (3' part of the fourth intron of lin-3) is
in lower case letters and the exon sequence (5' part of the fourth exon
of lin-3) is in upper case letters (purple underline). The
e1417 mutation, indicated with a dot, is located in the conserved
region of the FTZ-F1 NHR binding site, underlined in green. E boxes are
underlined in red. Binding sites of POU homeodomain proteins are underlined in
blue. The unbroken blue line indicates the site conserved in both species; the
broken blue line represents the site that exists only in C.
elegans.
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