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Fig. 3. Deletion and site-directed mutagenesis analyses of ACEL. (A) Deletion
analysis of ACEL. Deletions from the 5' end of the ACEL were made with
the endogenous lin-3 promoter (constructs 1-4). Deletions from the
5' or the 3' end of the ACEL were generated by PCR and the DNA
fragments were fused with a 
pes-10::gfp enhancer assay vector
(constructs 5-8). (B) Site-directed mutagenesis analysis of ACEL. Mutations in
the ACEL were generated using PCR and the PCR products were cloned into the
pes-10::gfp vector. Construct 1 has no mutations and the
others (2-18) have changes in the ACEL as indicated. For each construct, about
30 animals that express gfp in the tail were examined for
gfp expression in the AC.
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