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Fig. 5. The hlh-2 and nhr genes are involved in vulval induction and the expression of lin-3::gfp in the AC. (A-D) Defective lin-3::gfp expression in the AC was caused by soaking animals in the dsRNA solution against hlh-2. Transgenic animals that contain lin-3::gfp extrachromosomal arrays were soaked in the control dsRNA solution (RNA synthesized from blank vectors) and in the dsRNA solution against hlh-2. (B,D) GFP expression in the AC was examined in the animals that express gfp in pharynx at the early L4 stage. (E-G) Defects in vulval induction and vulval-uterine connection in animals treated with dsRNA against hlh-2. (E) Wild-type vulvae with control RNAi. (F) Defective vulval induction after hlh-2 RNAi. (G) Defective vulval-uterine connection after hlh-2 RNAi. (H-J) Defects in vulval induction and vulval-uterine connection in animals treated with dsRNA against nhr-25. (H) Defective vulval induction after nhr-25 RNAi. (I,J) Defective vulval-uterine connection after nhr-25 RNAi.





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