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Fig. 4. Activation of basal lamina degradation by MEE cell death stimuli.
Individual palate shelves were cultured without contact in the presence of
retinoic acid (RA; a MEE cell death activator) or staurosporine (a
broad-spectrum cell death activator). At the end of culture (10 hours), cell
death (green) and laminin (red) were detected in the same palate slice by
TUNEL and specific immunohistochemistry, respectively. RA induced extensive
cell death in the MEE and rugae (r) of isolated shelves, but basal lamina
degraded only in the MEE apoptotic region (arrows). Treatment with z-VAD
blocked RA-induced cell death and basal lamina remained intact. Generalized
induction of epithelial cell death with staurosporine activated the
degradation of the basal lamina underlying the dying MEE cells (arrowheads),
whereas basal lamina underlying the MEE adjacent epithelium (left from the
arrow) was unaffected. These experiments were repeated more than three times
at least in triplicate for each condition. Scale bar: 100 µm.
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