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Fig. 2. XMeis3-expressing cells caudalize adjacent anterior neural fated cells. XMeis3-injected (1.0 ng RNA) AC explants were taken from pigmented embryos and recombined with AC explants removed from albino embryos neuralized with the BMP DNR (0.2 ng RNA). In situ hybridization was performed to the otx2 (forebrain) and Krox20 (hindbrain) markers. (A) AC/BMP DNR explants. Expression of otx2 is high (n=7/12) in the neuralized albino side of these recombinant explants. (B) XMeis3/BMP DNR explants. Expression of otx2 is strongly inhibited (80%, n=11/14) in the albino side, and these cells undergo elongation. Notice that residual otx2 expression is detected at the distal end of the elongated explants and high expression is detected in non-elongated explants (14%, 2/14, asterisks). (C) AC/BMP DNR explants. No elongation or Krox20 expression is detected (n=0/6) on the albino side. (D) XMeis3/BMP DNR explants. Krox20 expression (67%, n=8/12) is induced in the elongating albino side. (E) In some elongating albino explants, two Krox20 stripes are detected, similar to the endogenous r3/r5 pattern in the embryo. (F,G) XMeis3/BMP DNR explants in which N17Ras- (1.0 ng) and BMP-DNR-encoding RNAs were co-injected on the albino side. In these explants, elongation is inhibited. (F) Expression of otx2 is rescued (100%; n=9/9). (G) Krox20 expression is inhibited in all of the explants (n=12/12).

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