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Fig. 4. XMeis3 induces convergent extension and membrane localization of dsh protein in recombinant explants. (A) AC/AC recombinant explant in which the albino side is injected with RNA (50 pg) encoding membrane-localized GFP. (B) DLMZ albino explant injected with membrane-localized GFP. The explant has undergone convergent extension and cells are intercalated. (C,D) XMeis3/BMP DNR recombinant explants in which the elongated BMP DNR albino side is co-injected with membrane-localized GFP. Cells in the elongated side have intercalated and undergone convergent extension in comparison to control explants (A). (E) AC/BMP DNR recombinant explant (late neurula) injected with RNA encoding dsh-GFP (50 pg) on the albino side (rhodamine detection). The dsh-GFP is detected diffusely throughout the cells in the non-elongating explant. (F) AC/BMP DNR recombinant explant (early neurula, pre-elongation) injected with dsh-GFP on the albino side (HRP detection). The dsh-GFP is detected diffusely throughout the cells in the explant. (G) XMeis3/BMP DNR recombinant explant (late neurula) injected with dsh-GFP on the albino side (rhodamine detection). The dsh-GFP is localized to the intercalated elongating cells. (H) XMeis3/BMP DNR recombinant explant (early neurula, pre-elongation) injected with dsh-GFP on the albino side (HRP detection). The dsh-GFP is membrane localized. (I) XMeis3/BMP + FGFI DNR recombinant explant (late neurula) injected with dsh-GFP on the albino side (rhodamine detection). The dsh-GFP is detected diffusely throughout the cells in the non-elongating explant. (J) XMeis3/BMP + FGFI DNR recombinant explant (early neurula, pre-elongation) injected with dsh-GFP on the albino side (HRP detection). The dsh-GFP is detected diffusely throughout the cells in the explant.

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