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Fig. 6. Inhibition of Wnt PCP activity inhibits XMeis3-induced convergent extension
but not neural marker expression. (A) XMeis3/BMP DNR recombinant explants in
which the neuralized albino cells undergo elongation (60%, n=14/24).
(B) XMeis3/BMP DNR explants co-injected in the albino explant with 0.5 ng of
wnt11 dominant-negative ligand (14% weak elongation,
n=2/24), (C) Xdsh-(d2) (22% weak elongation,
n=4/18) or (D) Xdd1 (no elongations, n=0/18)
encoding RNAs. Elongation is inhibited in the neuralized albino side in all of
the co-injected groups (B-D). (E) One-cell-stage embryos were injected in the
animal hemisphere with 1.0 ng of XMeis3 RNA, 0.5 ng of wnt11
dominant-negative ligand RNA or both. 18 animal cap explants were removed from
uninjected and injected groups of blastula embryos (stage 8-9). Explants from
each group were grown to stage 20 and total RNA was isolated. RT-PCR analysis
was performed with the markers Krox20 and HoxD1. EF1
served as a control to quantify RNA levels in the different samples. For
controls, RT-PCR and –RT-PCR were performed on total RNA isolated from
normal embryos.
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