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Fig. 1. Lethal phenotypes of rig mutant larvae. (A) The majority of
rig mutant larvae
(l(2)k07839/Df(2R)exu1) die during the third
instar and become stiff and extended in an apparent attempt to pupariate. (B)
rig mutants that die as prolonged second instar larvae reach the size
of a normal late third instar larva. The anterior spiracles of these prolonged
second instar larvae end with a single club-like structure (arrow) rather than
the multiple branches associated with a wild type third instar anterior
spiracle (F, arrow). (C) Two cuticles are often seen when rig mutants
die while molting. This larva died trapped inside the second instar cuticle,
which had separated almost completely from the third instar cuticle
(arrowhead). (D) This larva managed to shed its second instar cuticle from the
anterior portion of its body, but died while trying to shed this cuticle
further. A thin piece of cuticle extends from its mouthhooks to the point on
the body where the second instar cuticle remains (arrowhead). (E,F) A y
w early second instar larva (E) and y w late third instar larva
(F) are shown as controls. All panels are lateral views with anterior to the
right, except for E which is a dorsal view. All images were taken at the same
magnification.
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