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Fig. 10. VCAM1 and FLK1 in isolated whole allantoises and allantoic subregions after
8 hours of culture. Whole allantoises and allantoic subregions were placed
into the exocoelomic cavity or in isolation in test tubes and cultured for 8
hours, after which they were VCAM1 (A-G) or FLK1 immunostained (H-J) (brown
color). Allantoises from wild-type unoperated cultured (D,G) and/or
lacZ/+ ex vivo conceptuses (C) were used as controls. Sections were
counterstained in nuclear fast red (A-D,H-J) or hematoxylin (E-G). In A-B, E-F
and H-J, stages of synchronous donor allantoises and host conceptuses before
and after culture are separated by a `/'. In D,G, stages of whole conceptuses
before and after culture are separated by `/'. An ex vivo allantois is
contained in C. (A) VCAM1 in distal region of whole X-gal-stained allantois in
normal orientation. (B) VCAM1 is maintained in the distal region of a reversed
polarity whole X-gal-stained allantois. The VCAM1-negative proximal region has
made contact with the chorion. (C) Normal distal VCAM1 in X-gal-stained
lacZ/+ ex vivo control conceptus from the same experiment as A,B. (D)
Wild-type unoperated cultured conceptus from same experiment as A-C shows
normal distal VCAM1 in the allantois. (E-G) Allantoises are from wild-type
conceptuses. (E) Proximal allantoic third does not contain VCAM1. (F) Distal
two-thirds of the same allantois in E contains VCAM1. (G) Unoperated cultured
control conceptus from same experiment as E,F. (H-J) Whole X-gal-stained
allantois (H), X-gal-stained distal two-thirds (I), and X-gal-stained proximal
third (J) of the same allantois in I exhibit FLK1 throughout the allantoic
core. Abbreviations as in Figs
1,
2,
8. Scale bar in J: 50 µm
(E,F); 75 µm (A,H-J); 100 µm (B-D,G).
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