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Fig. 6. Effects of epidermal-specific deletion of Bmpr1a on expression of regulators of hair shaft and IRS differentiation. (A-F) In situ hybridization of mid-dorsal skin from K14-Cre40; Bmpr1a^cl/+ control littermate (A,C,E) and K14-Cre40; Bmpr1a^cl/cl mutant (B,D,F) at P8, using the digoxigenin-labeled antisense probes indicated. Hybridization signals (purple-brown) are indicated by arrows. Dark brown cells (A,E,F) are pigmented. (G,H) Immunofluorescence of P12 dorsal K14-Cre40; Bmpr1a^cl/+ control littermate (G) and K14-Cre40; Bmpr1a^cl/cl mutant (H) skin with anti-GATA3. (I,J) In situ hybridization of ventral head skin from K14-Cre40; Bmpr1a^cl/+ control littermate (I) and K14-Cre40; Bmpr1a^cl/cl mutant (J) at P8 using ³⁵S-labeled antisense probe for Msx1. (K,L) In situ hybridization of mid-ventral P8 skin from bcre-32; Bmpr1a^cl/+ control littermate (K) and bcre-32; Bmpr1a^cl/null mutant (L) using ³⁵S-labeled antisense probe for Lef1. Bright red regions (K; white arrows) are due to reflection of light by the hair shaft. (M,N) Immunofluorescence for ß-catenin (red) in P12 dorsal skin from K14-Cre40; Bmpr1a^cl/+ control littermate (M) and K14-Cre40; Bmpr1a^cl/cl mutant (N). Arrows (A,C,E,F,I-L) indicate hybridization signals. Arrows (G,M,N) indicate nuclear localized GATA3 and ß-catenin, respectively. Nuclei are counterstained with Hoechst 33258 (I-N). Scale bar in N applies to A-N. DP, dermal papilla.

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