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Fig. 3. Opa is required for Runt-dependent activation. Expression of slp1 mRNA in embryos that are wild-type for runt (A,B), or that have high levels of NGT-driven Runt (D,E). Ectopic Runt in these embryos was obtained by crossing females heterozygous for both NGT40 and NGTA to homozygous UAS-Runt[232] males. The embryos in A and D are wild type for opa, whereas the embryos in B and E are homozygous for the opa[1] mutation. (C) Schematic interpretation of the response of slp1 to the loss of Opa in an embryo with normal Runt expression. (F) Schematic interpretation of the effects of loss of Opa in an embryo with uniform Runt expression.





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