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Fig. 2. (A) Representation of the domain of Xbh1 expression in comparison
with those of other genes used as morphogenetic landmarks of different regions
of the CMZ and central retina. (B) Xbh1 is positively regulated by
Xath3 and Xath5, but not by XneuroD. RT-PCR was
performed on animal caps injected with 1 ng of either Xath3 or
Xath5 mRNA, or with 500 pg of XneuroD mRNA. Uninjected
animal caps (uninj. caps) and stage 28 whole embryos (WE st. 28) were used as
negative and positive controls, respectively; Xbrn3.0 and
Xbrn3d were used as positive control markers. (C) Xbh1 mRNA
injection (1 ng) is able to activate Xbrn3 genes and Xath3, but not
Xath5.
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