|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 6. Competition and interaction of Sox8 and Sox10 on bona fide binding sites
from target gene promoters. (A) Electrophoretic mobility shift assays with the
Sox10-binding sites 1-3 from the Mbp promoter
(Stolt et al., 2002) as
probes, and extracts from transfected N2A cells-expressing full-length Sox8
and Sox10 proteins as indicated below the lanes. Antibodies directed against
Sox8 (
-Sox8) or Sox10 (-Sox10) were added to some reactions
during the incubation period as indicated. m, bound monomer; d, bound
homodimer. Supershifted complexes are marked with an asterisk. (B)
Electrophoretic mobility shift assay with site 2 from the Mbp
promoter as probe and Sox8-containing N2A cell extract as protein source.
Increasing amounts of a truncated Sox10 (MIC variant) (see
Kuhlbrodt et al., 1998b;
Schlierf et al., 2002) were
added to the reactions as indicated. (C) Electrophoretic mobility shift assay
with the prototypic dimeric Sox10-binding site C/C'
(Peirano and Wegner, 2000) and
extracts from transfected N2A cells expressing a short version of Sox8 and a
long version of Sox10 either alone or in combination as indicated below the
lanes. The heterodimer is indicated by an arrowhead.
|
