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Fig. 4. Analysis of Notch/Su(H) regulation of a minimal twist promoter. Lateral views of stage 10 embryos stained with anti-GFP. (A,C,E,G) Whole-mount embryos. (B,D,F,H) Corresponding closeups of embryos in A,C,E,G. (A,B) 1428twist embryo exhibits low and high GFP domains. Notice that high domains appear chevron-shaped. (C,D) 1428twist; UAS-N^intra embryo has narrower high GFP domains than 1428twist embryos. GFP expression is repressed so that high GFP domains appear triangle-like in 1428twist; UAS-N^intra embryos. (E,F) 1428twist^mutSu(H) embryo displays a modulated pattern of low and high GFP domains. Uniform high GFP expression is not maintained throughout the mesoderm. Additionally, compared with 1428twist, high GFP domains appear to be slightly expanded. (G,H) 1428twist^mutSu(H); UAS-N^intra embryo looks different from the three embryos shown above. GFP expression in presumptive high GFP domains, especially laterally, is repressed compared with 1428twist embryos, so 1428twist^mutSu(H); UAS-N^intra high GFP domains appear most similar to those seen in 1428twist; UAS-N^intra embryos. However, at the same time, compared with 1428twist embryos, some cells in the presumptive low GFP domains of 1428twist^mutSu(H); UAS-N^intra embryos express GFP at high levels; this phenotype is most similar to that of 1428twist^mutSu(H) embryos.

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