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Fig. 6. Da activity regulates Twist expression. Lateral views of stage 8 (A,B) and stage 10 (C-H) embryos stained with anti-Twist. (A,B,C,E,G) Whole-mount embryos. (D,F,H) Corresponding closeups of embryos in C,E,G. (A) Wild-type (wt) stage 8 embryo expresses Twist uniformly at high levels throughout the mesoderm. (B) da¹ embryo, a mutant with reduced maternal and zygotic Da levels, expresses Twist at low levels at stage 8, as well as at later stages (data not shown) (Castanon et al., 2001). (C,D) UAS-da embryo, panmesodermally expressing high levels of Da, ectopically expresses high levels of Twist, similar to N^null mutants. (E,F) UAS-da embryo, panmesodermally expressing lower levels of Da than the embryo in C-D, ectopically expresses high levels of Twist, but shows a milder phenotype than the embryo in C-D. Black arrowhead indicates cells, located in what should be the low Twist domain, that express higher amounts of Twist than wild type. (G,H) UAS-da-da embryo, panmesodermally expressing the linked Da transgene under the same GAL4 conditions as the embryo in E-F, maintains uniform high Twist expression throughout its mesoderm similar to N^null mutants (see Fig. 1E,F).

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