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Fig. 2. The progressive stages in the morphogenesis of hollow acini-like structures
that are generated by culturing MCF-10A immortalized mammary epithelial cells
in basement membrane gels (Matrigel). Confocal images show solid masses at day
5 (left), structures with centrally localized apoptotic cells at day 10
(middle) and hollow structures at day 15 (right). Apoptotic cells were
identified using an antibody to caspase 3 (green); laminin 5 was detected with
an antibody to the g2 laminin chain (red); and nuclei were stained with DAPI
(blue). Images courtesy of Kenna R. Mills and Jay Debnath (Brugge's
Laboratory, Harvard Medical School, Boston, MA, USA).
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