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Fig. 1. Aberrant expression of Hh target genes in igu mutants and
comparison with four other Hh signaling mutants. (A1-A7) Wild-type, (B1-B7)
igu, (C1-C7) syu/shh, (D1-7) dtr/gli1, (E1-E7)
yot/gli2 and (F1-F7) con embryos at 30 hpf. (A1-F1,A2-F2)
Expression of nk2.2 in the ventral neural tube is reduced in all
mutants. Only igu embryos retain a punctate nk2.2 expression
(arrowheads in B1, inset is a higher magnification of boxed area). Lateral
views are shown in A1-F1 and the respective cross-sections at the middle of
the yolk extension are shown in A2-F2. (A3-F3) Expression of pax6 is
ventrally expanded in yot/gli2 (E3) and con (F3), but not in
other mutants. (A4-F4,A5-F5) Expression of en1 in somites is shown in
lateral views and in cross-sections. en1 expression in MPs and MFFs
is completely lost in con (F4, F5). A low level of expression
persists in syu/shh (C4) and yot/gli2 (E4), and no defect of
en1 expression is seen in dtr/gli1 mutants (D4,D5). In
contrast to the other four mutants, en1 expression in somites is
upregulated in igu embryos (B4,B5). (A6-F7,A7-F7) Expression of
ptc1 is shown in whole mount and in cross-sections. Expression of
ptc1 is reduced in syu/shh, dtr/gli1, yot/gli2 and
con mutants (C7-F7). Expression is most severely reduced in
con mutants, and is mildly reduced in the neural tube of syu/shh,
dtr/gli and yot/gli2 mutants. Note that ptc1 expression
is not maintained in somites of dtr/gli1 embryos, despite the
presence of en1 expression, and that the ptc1 expression
persists in the sclerotome regions of yot/gli2 (E7). In igu
embryos, ptc1 expression is upregulated in the entire somites (B6,
B7), whereas the expression in neural tube is unaffected or slightly reduced
in the trunk, and slightly more reduced in the brain.
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