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Fig. 5. Activation of PKA does not efficiently suppress Hh target gene expression. (A-D) In wild-type embryos, the expression of myod at 14 hpf (A), nk2.2 at 30 hpf (B), en1 at 30 hpf (C) and ptc1 at 30 hpf (D) is repressed by 75 µM forskolin treatment. (E-H) In igu embryos, treatment with 75 µM forskolin did not block Hh target gene expression, with expression patterns similar to those seen in untreated igu mutants. Genotypes were confirmed by PCR after in situ analysis.





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