|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 8. Rescue of igu mutant phenotype by igu/dzip1 mRNA
and permissive nature of Igu/Dzip1 proteins. (A-J) Injection of wild-type
igu/dzip1 mRNA. Nearly all igu embryos injected with
wild-type igu/dzip1 mRNA completely recovered the normal expression
of Hh target genes (A-E), including: ptc1 expression at 14 hpf (A),
nk2.2 expression at 30 hpf (B), ptc1 expression at 30 hpf
(C, D) and en1 at 30 hpf (E) (compare with
Fig. 1). Wild-type embryos
injected with wild type igu/dzip1 mRNA did not show any defects in Hh
target gene expression (F-J). (K-T) Injection of mutant mRNA that encodes
truncated igu/dzip1 proteins. Expression of mutant Igu/Dzip1 does not
affect Hh target gene expression in igu mutants (K-O) or wild-type
embryos (P-T). (A,D,F,I,K,N,P,S) Dorsal views and other panels are side views.
Expression patterns in A-J are identical to those seen in wild-type embryos,
while those in K-O are identical to igu mutant embryos (see
Fig. 1 for comparison).
|
