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Fig. 1. Structure of the transgene insertion site in the doubleridge mutant. (A) Sequence of the two junction fragments from overlapping cosmid clones. (B) Position of the mouse genomic sequence in cosmid clones (black) aligned with the assembled genomic sequence of a 400-kb region of mouse chromosome 19. The position of the 60-kb doubleridge deletion and nearby genes is shown. Open box, noncoding exon; filled box, coding exon; arrow, direction of transcription. The promoter of Prkg1 is located at 31.3 Mb. (C) Confirmation of the 60-kb deletion in doubleridge genomic DNA. Primer pairs located between the cosmid junction sequences do not amplify doubleridge genomic DNA.





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