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Fig. 1. Cell positions in live Medaka embryos and their transfer to a computer
graphics model. (A) Two labeled cells in an embryo at stage 15++ (20 hpf).
(a,c) Fluorescence images of the same embryo viewed from different angles.
Broken white lines indicate outline of embryo, blastoderm edge and cell
thickening along the dorsal midline. Images in a,c were superimposed on stage-
and size-matched computer graphics models in b,d, and positions of the two
fluorescent cells were plotted on the model using red and black dots,
respectively. The diameter of dots corresponds to 20 µm, approximating
average cell size. Insets in a,b,d are enlargements of the squares indicated
by broken lines. The cell coordinates l1 (along the dorsal midline
shown by red broken line) and d1 (distance along the circumference
from the midline) of the first measurement (b) agreed with those of the second
(l2 and d2), and two plots overlapped well (d). (B) Three
cells at stage 17 (25 hpf). (a,c,e) Fluorescence images of dorsal, lateral and
frontal angles. (b,d,f) Cell positions in a,c,e are plotted on the computer
graphics model. Insets in a,b are enlargements of the small squares. The cells
indicated by the green dots later contributed to retina, while those indicted
by orange dots contributed to mesencephalon. Error bars indicate standard
deviations in embryo length along the dorsal midline and positions of the
plotted cells (insets). Scale bars: 200 µm.
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