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Fig. 3. Time course of cells labeled at stage 13 (13 hpf) and their descendants
until stage 24 (44 hpf). (A) Precursor cell populations at stage 13 and (C-E)
their descendants at stage 24. Throughout, cells are indicated by dots and
color-coded according to the location of cell fates at stage 24 as indicated
in (C): telencephalon (Tel, red); retina (Re, green); diencephalon (Di,
black); mesencephalon (Mes, orange); rhombencephalon (Rho, blue); spinal cord
(SC, white); epidermis (Epi, light blue); peripheral nervous system including
placodes and neural crest (yellow); trunk mesoderm (pink). Data of left and
right halves are superimposed assuming bilateral symmetry of the embryo. (A)
Plots of cells on the embryo model at stage 13 in animal pole view (dorsal
midline towards the bottom). Double-colored dots (e.g. arrowhead) in A
indicate cells that contributed to two different neural subdivisions or
tissues as shown by cells in Fig.
2A,B. A pair of cells located adjacent to one another at stage 13
are indicated by black and white arrows. These cells are indicated in the
panels of later embryos, and at stage 24 (D,E) are located distantly in the
diencephalon and rhombencephalon. (B) Outline of territories occupied by the
groups of cells later contributing to distinct neural subdivisions, which are
indicated by the same color codes as the cells. (C) Neural subdivisions of
Medaka at stage 24 illustrated with distinct colors. (D,E) Distribution of
labeled cells in stage 24 embryo in side (D) and dorsal (E) views. (F-K)
Distribution of color-coded cells on embryo model from stage 13 to stage 18.
(F) Stage 13 (early gastrula, 13 hpf); (G) stage 15 (mid-gastrula, 17.5 hpf);
(H) stage 16 (late gastrula, 21 hpf); (I) stage 16+ (late gastrula +, 22 hpf);
(J) stage 17 (25 hpf); (K) stage 19 (two somites, 28 hpf). Cell groups forming
individual neural subdivisions are clearly separated during the 1 hour period
21 hpf (stage 16) to 22 hpf (stage 16+). (A',G'-K')
Enlargement of squares in A,G-K. Scale bars: 200 µm. Black and white arrows
indicate two individual cells as in A-K
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