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Fig. 2. Immunolocalization of WRN-1 in C. elegans at various developmental stages. C. elegans embryos or worms were reacted with a mouse antiserum against WRN-1 (green) and then with an FITC-conjugated secondary antibody, followed by nuclear staining with DAPI (blue). (A) C. elegans embryos. Mitotic prophase nuclei, two nuclei in the 6-cell embryo and one nucleus in the 8-cell embryo, are marked with arrowheads; a metaphase nucleus in the 8-cell embryo is marked with an arrow. (B) L1 larval and adult stage worms. (C) Intestine and gonad from an L4 stage larva. (D) An oocyte and an embryonic metaphase cell. Scale bars: A, 10 µm; B, 50 µm; C, 50 µm; D, 5 µm for the oocyte and 1 µm for the metaphase cell.





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