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Fig. 7. Reduction of S-phase duration by wrn-1(RNAi) during embryogenesis.
(A) Accelerated cell division of a wrn-1(RNAi) embryo observed with
Nomarski optics at 25°C. (B) Time-lapse DIC microscopy of 2- to 4-cell
embryos. At 0 minutes, AB (right) and P1 (left) cells of a wild-type N2 embryo
immediately after cytokinesis are shown. Nuclear envelope breakdown (NEBD) of
AB and P1 cells occurs at 12 and 14 minutes, respectively. (C) Average
duration of S phase in AB and P1 determined by timing cytokinesis and NEBD.
The bar graphs correspond to the time separating cytokinesis of P0 from NEBD
of either the AB or the P1 cell. Standard errors (s.e.m.) are shown by error
bars. Ten embryos were observed for each estimate of S-phase duration. Scale
bars: A, 20 µm; B, 10 µm.
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