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Fig. 3. Cyclin E overexpression results in nuclear loss. RNAs encoding cyclin E1
alone (A) or cyclin E1 with cdk2 (D), cyclin A2 alone (B) or cyclin A2 with
cdk2 (E), cdk2 alone (C) or ß-gal as a control (F) were injected into one
cell of a 2-cell embryo and allowed to develop to stage 8.5. Embryos were
fixed, depigmented and the DNA stained with Hoechst. Animal caps were
dissected and viewed by fluorescence microscopy. White arrows indicate regions
in which nuclei are absent.