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Fig. 6. Commissure formation requires endocytosis. Frontal views of dissected
central nervous system (CNS) preparations of stage-16 embryos stained for the
presence of all CNS axons using Mab BP102. Anterior is up. (A) Wild-type
embryo. (B) Overexpression of schizo in all midline cells of a
heterozygous slit embryo leads to a fused commissure phenotype. (C)
Expression of a dominant negative form of Shibire in all CNS midline cells
blocks endocytosis and leads to a schizo-like CNS phenotype. (D)
Expression of a dominant negative form of Shibire in all CNS midline cells of
a heterozygous slit animal does not impair commissure formation. (E)
Expression of a dominant negative Arf6 protein in all CNS midline cells lead
to schizo phenocopy.
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