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Fig. 1. Genetic interactions between let-756, egl-15 and clr-1. (A) Wild type. (B) let-756 unc-32 arrested L1 larva. (C) clr-1(e2530) Clr hermaphrodite. (D) let-756 unc-32; ayIs25[egl-15(+)] Scr hermaphrodite. (E) ayIs26[P_hsp16-2::let-756]; ayIs25[egl-15(+)] Clr hermaphrodite. (F) ayIs26[P_hsp16-2::let-756]; soc-2; ayIs25[egl-15(+)] Soc hermaphrodite. (G) clr-1(e1745ts); let-756 unc-32 Scr hermaphrodite, at 15°C. (H) clr-1(e1745ts)/clr-1(e2530); let-756 unc-32 non-Clr non-Scr hermaphrodite, at 15°C. (I) clr-1(e2530); let-756 unc-32 viable semi-Clr hermaphrodite. All animals were photographed as adults. (E-F) Animals were heat shocked for 30 minutes at 37°C, and allowed to recover at 20°C for 5.5 hours. The Clr phenotype in (C) and (E) can be discerned from the appearance of the intestine floating within the fluid-filled pseudocoelomic cavity. Abbreviated genotypes: 0, null allele; ts, temperature-sensitive allele; ++, transgenic overexpression. Scale bar: 200 µm.

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