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Fig. 6. DNA content in prespore and prestalk cells of migrating slugs. (A)
Wild-type cells (AX4) marked with a prestalk reporter gene (ecmA/gfp)
were developed on agar to the slug stage and dissected into two fractions
enriched in anterior or posterior cells. (B) Cells were dissociated, fixed and
stained with propidium iodide to obtain population profiles of their DNA
content. (C) The cells were divided into GFP-negative (window A) and
GFP-positive (window B) populations that were confirmed by direct visual
inspection of sorted cells. The percentage of the total cell population in
each window is shown. (D) The DNA content profiles of the GFP-negative cells
in window A of the slug posteriors (upper panel) and the GFP-positive cells in
window B of the anterior cells (lower panel).
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