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Fig. 1. HTL is required for cell shape changes during mesoderm migration. Embryos expressing twi::CD2 were stained with anti-CD2 antibodies (red) and anti TWI antibodies (green) and either visualized as whole mounts after optical sectioning by confocal microscopy (D-F,J,K,L,N) or cross-sectioned after plastic embedding (A-C,G-I). (M) Wild-type embryo stained against NRT (red) and TWI (green). Scale bar: 20 µm for A-L. (A-F) Wild-type embryos. (A,D) Phase 1. (B,E) Phase 2; long cellular protrusions are marked with arrows in E. (C,F) Phase 3. (G-L) Embryos homozygously mutant for htl^AB42; (G,J) phase 1; note that some mesoderm cells are out of the focal plane, because they are not attached to the ectoderm (J). (H,K) Phase 2; note that cells remain rounded and no cell shape changes occur. (I,L) Phase 3; some cells extend protrusions (arrow in L). (M) Cross-section through the migrating mesodermal aggregate of a wild-type embryo during phase 2. The cells adopted a polarized morphology, with the front attached to the ectoderm and the rear attached to the neighboring cells. (N) htl^AB42 mutant embryo after mesoderm migration (stage 10). Note the formation of cellular protrusions of cells attached to the ectoderm (arrowheads).

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