QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 6. The function of PBL in mesoderm migration is mesoderm autonomous. Embryos hemizygous for pbl³, expressing twi::Gal4, UAS::pbl^3.² were stained with anti-NRT (red) and anti-TWI (green) antibodies and sectioned. Embryos are shown in phase 1 (A), phase 2 (B) and phase 3 (C) of migration. A complete rescue of cell shape change defects in pbl mutants is seen. Note that cytokinesis is occurring normally in the mesoderm while still blocked in the ectoderm (insert in B; the asterisks mark nuclei). (D,E) Differentiation of EVE-positive mesoderm cells is rescued by expression of UAS::pbl^3.2 with twi::Gal4 in pbl³ hemizygously mutant embryos. (D) Stage 11 embryo containing at least one wild-type copy of the pbl gene; the blue staining indicates presence of the TM3 (ftz::lacZ) chromosome. (E) Stage 11 embryo of the genotype twi::Gal4/UAS::pbl^3.2; pbl³/Df(3L)pbl^NR.

Return to article