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Fig. 7. Requirement of PBL for HTL-triggered cell shape changes. (A,C,E) Sections of embryos at stage 7 stained with anti-NRT (red) and anti TWI (green) antibodies. The contacts of the mesoderm with the ectoderm cells are marked with arrowheads in A,C. (B,D,F) Lateral surface views of embryos at stage 11 stained with anti-EVE antibodies. (A,B) htl^AB42 homozygous embryos overexpressing full-length HTL protein (twi::Gal4; htl^AB42, UAS::htl^M/htl^AB42). (C,D) htl^AB42 homozygous embryos overexpressing constitutively activated HTL (twi::Gal4; htl^AB42, UAS::htl/htl^AB42). (E-G) pbl³ hemizygous embryos overexpressing the activated form of HTL (twi::Gal4; pbl³, UAS::htl/Df(3L)pbl^NR); embryo in G is at stage 9. Arrowhead in E indicates the failure of mesoderm cells to contact the ectoderm. (H,I) Cross-sections of wild-type (H) and pbl³ homozygous mutant (I) embryo at stage 8 stained with anti-dpERK antibodies. Arrowheads indicate mesoderm cells at the leading edge that stain for dpERK.

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