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Fig. 1. Ectopic expression of Wnt7a promotes neuronal differentiation in cortical
NPCs. Dissociated cortical NPCs were infected with a retrovirus encoding
either GFP alone (control) or both GFP and Wnt7a. The cells were then
incubated for 3 days in the absence of Fgf2. (A) The percentage of
TuJ1+ cells among GFP+ cells was determined. Data are
the mean±s.e.m. of values from three samples. Similar results were
obtained in four independent experiments. *P<0.025
versus control; t-test. (B) GFP fluorescence, TuJ1 immunofluorescence
and the corresponding merged images are shown for typical fields of control
and Wnt7a-expressing cells. (C) The percentage of GFAP+ cells among
GFP+ cells was determined. Data are the mean±s.e.m. of
values from three samples. Scale bar: 25 µm.
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