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Fig. 2. Wnt7a promotes neuronal differentiation through the ß-catenin/TCF signaling pathway. (A) NPCs were infected with a retrovirus encoding GFP alone (control) or GFP together with the ß-catenin mutants S33Y or N90 or with either wild-type (WT) or a kinase-negative mutant (KN) of MKK7-JNK1. The percentage of TuJ1⁺ cells among GFP⁺ cells was determined after culture for 2 days in the absence of Fgf2. Data are the mean±s.e.m. of values from eight samples, and similar results were obtained in three independent experiments. (B) NPCs were infected with a retrovirus encoding GFP alone (control) or GFP together with S33Y ß-catenin. The percentage of neurofilament⁺ cells among GFP⁺ cells was determined after culture for 4 days in the absence of Fgf2. Data are the mean±s.e.m. of values from eight samples, and similar results were obtained in three independent experiments. (C) GFP fluorescence, neurofilament (NF) immunofluorescence and the corresponding merged images of control cells and cells expressing S33Y ß-catenin are shown. Arrowheads indicate neurite-like protrusions induced by the expression of S33Y ß-catenin. Scale bar: 25 µm. Data are the mean±s.e.m. of values from six samples, and similar results were obtained in three independent experiments.

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