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Fig. 4. Expression of a stabilized form of ß-catenin instructively promotes
neuronal differentiation. NPCs were infected with a retrovirus encoding either
GFP alone (control) or both GFP and S33Y ß-catenin at a low titer and
subjected to clonal analysis. After incubation for 3 days in the presence of
Fgf2, cells in each clone were stained with TuJ1 antibody and the clones were
classified as containing either only TuJ1+ cells (neuron-only
clone), both TuJ1+ and TuJ1- cells (neuron-containing
clone), or only TuJ1- cells (precursor-only clone) (A). The cell
number for each clone was determined (B). The results are representatives of
three independent experiments. (C) NPCs were infected with a retrovirus
encoding either GFP alone (control) or GFP together with Wnt7a, S33Y
ß-catenin or wild-type (WT) ß-catenin. They were then plated at a
density of 1000 cells/well in 96-well plates that had been coated with
poly-HEME and incubated for 9 days in suspension culture in the presence of
Fgf2, after which the number of GFP+ primary neurospheres per 1000
founding cells/well was determined. Data are the mean±s.e.m. of values
from 12 samples, and are representatives of three independent experiments.
*P<10-5 versus control.
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