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Fig. 6. Competence to respond to SHR::GFP-mediated cell fate changes.
Endodermis-specific markers. Casparian strip histochemical staining (A,C,E)
and JIM13 immunostaining (B,D,F) of transverse sections of wild-type (A,B),
pGL2::SHR::GFP (C,D) and pWER::SHR::GFP (E,F) transgenic
roots. C,D and E,F are consecutive sections (the star and the asterisk
indicate the same cell in C,D and E,F, respectively). In both transgenic
lines, Casparian strip is detected in the normal location of the endodermis
(arrowheads in A,C,E), as well as in some cells of the outermost tissue
(arrows in C,E). Lignin in the xylem is also stained. JIM13 is also detected
in the normal location of the endodermis (B,D,F) and in some cells of the
outermost tissue (D,F). Some cells in the stele are also stained by JIM13. ep,
epidermis; co, cortex; en, endodermis; st, stele. Scale bars: 25 µm.
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