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Fig. 6. Phenotypes of Prs- mutant Arabidopsis leaves and
cotyledons. At maturity non-mutant (A) and Prs- mutant (E) rosette
leaves have equivalent phenotypes. Whole-mount (B,C,F,G) and scanning electron
microscopic (D,H) analyses of non-mutant (B-D) and Prs- mutant
(F-H) leaf primordia (L1, L2) reveal that the lateral stipules (S) are deleted
from mutant leaves. Note that the size of the emerging trichomes (T) indicates
that the lack of stipule development in Prs- mutant primordia is
not due to differences in the developmental age of these samples. No obvious
Prs- mutant phenotype is noted in comparisons of mature (I,J) or
primordial (not shown) non-mutant (I) and Prs- mutant (J)
cotyledons. (K-M) Expression of PRS during Arabidopsis
embryogenesis. (K) PRS expression is localized to the tips of the
prospective cotyledons in the heart stage embryo. In torpedo stage embryos,
PRS is expressed at the apices (L) and the lateral margins (M) of the
cotyledons (c). PRS-expressing cell layers therefore define a border
between adaxial and abaxial side of the cotyledons, similar to the maize
coleoptile (Fig. 4B). Scale
bars: 50 µm.
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