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Fig. 1. Expression of Cdc42, Rac1 and Rhoa/b in mouse at
embryonic day 11-13. At E11, Cdc42, Rac1 and Rhoa expression
was located in the ventricular (vz in A; short arrows in A',C,E) and
subventricular zone (svz in A; large arrows in A',C,E), whereas
Rhob transcripts were present in the early migratory stream in the
marginal zone (mz in A; short arrows in G,G'), as well as in the
submarginal zone (smz in A; large arrows in G,G'). At E13,
Cdc42, Rac1 and Rhoa/b genes were strongly expressed by
migrating LRN located in the marginal stream (arrowheads in B,D,F,H). The ION
(asterisks in B,D,F,H) showed strong levels of Cdc42 and
Rhob expression (B,H), and lower levels of Rac1 and
Rhoa expression (D,F). In addition, all four transcripts were present
in the developing floor plate (fp; A-H). The roof plate (rp) at E11-E13 and
the ventral ventricular zone of the hindbrain at E13 (arrows in B,D,F) showed
different levels of Cdc42, Rac1 and RhoA expression. Note
the presence of Rhob transcripts in the hypoglossal nucleus (XII;
G,H). (I,J) Western blot illustrating the presence of Cdc42, Rac1 and RhoA
proteins in rhombic lip at E11 (delimited ventrally by dotted line in A) and
E12 (I), as well as in the LRN and ION domains (into the dotted square in B)
at E13 (J). (K) Schematic drawings of the spatio-temporal changes in the
Cdc42, Rac1 and Rhoa/b expression at E11 and E13. Scale
bars: in A, 200 µm for A,C,E,G; in A', 140 µm; in B, 400 µm for
B,D,F,H.
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