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Fig. 3. RhoA/B/C GTPases are strictly required for nuclear migration to occur. (A) RhoA modification by TAT-C3 through ADP-ribosylation at E11. In vitro ADP-ribosylation of explants pre-treated with TAT-C3 showed that the radiolabeled Rho band was markedly decreased, indicating that TAT-C3 efficiently modified Rho substrate in neurons. (B,C) When E11 rhombic lip explants were treated with 20 µg/ml TAT-C3, neurites lost their preferential orientation toward the netrin 1 (Net) source (B) and cell nuclei failed to translocate within those neurites (C). (D,E) Quantification analysis of (D) migration (cumulative distributions and histograms) and (E) axon outgrowth (n=13 in TAT-C3-treated explants; n=19 in control explants). (F) Migration/ougrowth ratio of control and TAT-C3-treated explants. Error bars in D, E and F represent s.e.m. *P< 0.001. Scale bars: B, 500 µm; C, 200 µm.





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